Subcutaneous or Oral Immunization of Mice with <i>Lactococcus lactis</i> Expressing F4 Fimbrial Adhesin FaeG
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چکیده
منابع مشابه
Immunogenicity of recombinant F4 (K88) fimbrial adhesin FaeG expressed in tobacco chloroplast.
To test the possibility of producing the novel vaccine in plants against diarrhea normally found in neonatal and newly weaned piglets, the faeG gene, encoding a major F4ac fimbrial subunit protein, was introduced into the tobacco chloroplast genome. After two rounds of selection under spectinomycin, we obtained the transgenic plants nearly homoplasmic. RNA gel blot analysis indicated that faeG ...
متن کاملCholera toxin improves the F4(K88)-specific immune response following oral immunization of pigs with recombinant FaeG.
Oral immunization of both humans and animals with non-replicating soluble antigens often results in the induction of oral tolerance. However, receptor-dependent uptake of orally administered soluble antigens can lead to the induction of an antigen-specific immune response. Indeed, oral immunization of pigs with recombinant FaeG (rFaeG), the adhesin of the F4(K88) fimbriae of enterotoxigenic Esc...
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Lily pollen was developed to express HBsAg by Agrobacterium-mediated transformation. A double prime-boost strategy combining parenteral and oral HBsAg boosters was found to increase antibody titer levels 10-fold, as compared to a single process of priming and boosting. Lily pollen may prove a tool for oral vaccine development.
متن کاملThe polymeric stability of the Escherichia coli F4 (K88) fimbriae enhances its mucosal immunogenicity following oral immunization.
Only a few vaccines are commercially available against intestinal infections since the induction of a protective intestinal immune response is difficult to achieve. For instance, oral administration of most proteins results in oral tolerance instead of an antigen-specific immune response. We have shown before that as a result of oral immunization of piglets with F4 fimbriae purified from pathog...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2013
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.12-0267